rabbit polyclonal anti human ccr5 antibody  (Novus Biologicals)

Journal: The Journal of Reproduction and Development

Article Title: Expression of C–C motif chemokines and their receptors in bovine placentomes at spontaneous and induced parturition

doi: 10.1262/jrd.2019-113

Figure Lengend Snippet: Transcripts upregulated and downregulated in caruncle tissue at spontaneous parturition compared with induced (DEX group) parturition

Article Snippet: The 5 μm-thick sections from formalin-fixed and paraffin-embedded tissue were incubated at room temperature with rabbit polyclonal anti-bovine CCL8 antibody (MBS2026335; 1:400; MyBioSource, San Diego, CA), rabbit polyclonal anti-human CCR1 antibody (ab140756; 1:200; Abcam PLC, Cambridge, UK), rabbit polyclonal anti-human CCR2 antibody (NBP1-48337; 1:100; Novus Biologicals LLC, Littleton, CO), or rabbit polyclonal anti-human CCR5 antibody (NBP2-31374SS; 1:100; Novus Biologicals LLC) for 12 h. The signals were detected using anti-rabbit IgG-biotin conjugate (Sigma-Aldrich, St. Louis, MO, USA) diluted 1:100 for 1 h and then counterstained with hematoxylin.

Techniques:

Journal: The Journal of Reproduction and Development

Article Title: Expression of C–C motif chemokines and their receptors in bovine placentomes at spontaneous and induced parturition

doi: 10.1262/jrd.2019-113

Figure Lengend Snippet: mRNA expression levels of CCLs and their receptors in the placentome at spontaneous (SP group) and induced (PG, DEX, and TABET groups) parturition. A, CCL2 ; B, CCL8 ; C, CCR1 ; and D, CCR5 mRNA expression. Statistically significant differences in the relative abundance of CCL2 , CCL8 , CCR1 , and CCR5 mRNA were analyzed in cotyledon (COT) and caruncle (CAR) tissue. Data are presented as the mean ± standard error. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The 5 μm-thick sections from formalin-fixed and paraffin-embedded tissue were incubated at room temperature with rabbit polyclonal anti-bovine CCL8 antibody (MBS2026335; 1:400; MyBioSource, San Diego, CA), rabbit polyclonal anti-human CCR1 antibody (ab140756; 1:200; Abcam PLC, Cambridge, UK), rabbit polyclonal anti-human CCR2 antibody (NBP1-48337; 1:100; Novus Biologicals LLC, Littleton, CO), or rabbit polyclonal anti-human CCR5 antibody (NBP2-31374SS; 1:100; Novus Biologicals LLC) for 12 h. The signals were detected using anti-rabbit IgG-biotin conjugate (Sigma-Aldrich, St. Louis, MO, USA) diluted 1:100 for 1 h and then counterstained with hematoxylin.

Techniques: Expressing

Journal: The Journal of Reproduction and Development

Article Title: Expression of C–C motif chemokines and their receptors in bovine placentomes at spontaneous and induced parturition

doi: 10.1262/jrd.2019-113

Figure Lengend Snippet: Primer sequences for PCR

Article Snippet: The 5 μm-thick sections from formalin-fixed and paraffin-embedded tissue were incubated at room temperature with rabbit polyclonal anti-bovine CCL8 antibody (MBS2026335; 1:400; MyBioSource, San Diego, CA), rabbit polyclonal anti-human CCR1 antibody (ab140756; 1:200; Abcam PLC, Cambridge, UK), rabbit polyclonal anti-human CCR2 antibody (NBP1-48337; 1:100; Novus Biologicals LLC, Littleton, CO), or rabbit polyclonal anti-human CCR5 antibody (NBP2-31374SS; 1:100; Novus Biologicals LLC) for 12 h. The signals were detected using anti-rabbit IgG-biotin conjugate (Sigma-Aldrich, St. Louis, MO, USA) diluted 1:100 for 1 h and then counterstained with hematoxylin.

Techniques: Sequencing

Journal: Discover Oncology

Article Title: CCR5 expression and conformational stability as potential cooperative modulators of immune phenotypes and therapy response in breast cancer

doi: 10.1007/s12672-025-04189-1

Figure Lengend Snippet: Comprehensive characterization of CCR5 expression, predictive significance, functional pathways, and immune contexture in breast cancer. A Expression distribution of CCR5 across normal human tissues. B Multivariate logistic regression analysis showing that high CCR5 expression is independently associated with reduced likelihood of achieving pathological complete response (pCR) after neoadjuvant chemotherapy (NAC). C Sensitivity analysis using a data-augmented cohort confirms the negative association between CCR5 and pCR, with a narrower confidence interval. D Gene Ontology (GO) gene set enrichment analysis (GSEA) reveals that CCR5-high tumors are enriched in immune-related processes including T cell activation and leukocyte adhesion, as well as cellular transport and metabolic pathways. E Hallmark GSEA indicates concurrent activation of pro-inflammatory pathways (e.g., IFN-γ response, TNFα–NF-κB, IL6–JAK–STAT3) and tumor-promoting programs such as epithelial-mesenchymal transition (EMT) and mTORC1 signaling. F KEGG pathway enrichment highlights both immunostimulatory (e.g., NK cell cytotoxicity) and protumorigenic (e.g., PI3K–Akt signaling) pathways in CCR5-high tumors. G Immune cell infiltration analysis based on multiple algorithms shows that CCR5-high tumors are associated with elevated CD8⁺ T cells, M1 macrophages, and activated dendritic cells, but also with immunosuppressive Tregs, M2 macrophages, and high stromal/immune scores, suggesting a biphasic immune microenvironment

Article Snippet: For CCR5 protein detection, we performed IHC using a rabbit anti-CCR5 polyclonal antibody (Proteintech#17476-1-AP, 1:900 dilution).

Techniques: Expressing, Functional Assay, Activation Assay, Protein-Protein interactions

Journal: Discover Oncology

Article Title: CCR5 expression and conformational stability as potential cooperative modulators of immune phenotypes and therapy response in breast cancer

doi: 10.1007/s12672-025-04189-1

Figure Lengend Snippet: Correlation between CCR5 expression and gene signatures of tumor-associated macrophage (TAM) polarization in breast cancer. A Correlation heatmap showing weak-to-moderate associations between CCR5 and M1 macrophage markers (e.g., NOS2, ARG2, PTGS2). B CCR5 expression demonstrates strong positive correlations with canonical M2 macrophage markers, including MRC1 ( r = 0.65), CD163 ( r = 0.699), and MS4A4A ( r = 0.749). C CCR5 is also highly correlated with TAM-associated immunosuppressive genes such as CD86, CCL22, and IL10, indicating a preferential link to immunoregulatory macrophage phenotypes

Article Snippet: For CCR5 protein detection, we performed IHC using a rabbit anti-CCR5 polyclonal antibody (Proteintech#17476-1-AP, 1:900 dilution).

Techniques: Expressing

Journal: Discover Oncology

Article Title: CCR5 expression and conformational stability as potential cooperative modulators of immune phenotypes and therapy response in breast cancer

doi: 10.1007/s12672-025-04189-1

Figure Lengend Snippet: Structural confidence and stability changes of CCR5 protein with V131I mutation ( A ) AlphaFold-predicted 3D structure of CCR5 highlighting residue 131. Confidence levels are color-coded based on pLDDT scores: very low (< 50), low (50–70), high (70–90), and very high (> 90). B – C Root Mean Square Fluctuation (RMSF) and Root Mean Square Deviation (RMSD) plots comparing wild-type (WT) and V131I mutant (MT) CCR5 during 100 ns molecular dynamics simulations. D Radius of gyration (Rg) trajectory indicating global compactness differences between WT and MT. E Solvent-accessible surface area (SASA) profile across simulation time. F Predicted change in protein stability (ΔΔG) upon V131I substitution at position 131, visualized as a heatmap. Red shading denotes destabilizing mutations

Article Snippet: For CCR5 protein detection, we performed IHC using a rabbit anti-CCR5 polyclonal antibody (Proteintech#17476-1-AP, 1:900 dilution).

Techniques: Mutagenesis, Residue, Solvent